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KMID : 0613820180280050532
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Journal of Life Science
2018 Volume.28 No. 5 p.532 ~ p.539
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Effect of Black Rice (Oryza sativa L. indica) Ethanolic Extract on Tyrosinase Activity and Antioxidant Activity Related to Melanin Production
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Lee Eun-Been
Kim Moon-Moo
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Abstract
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Hair graying is processed by loss of melanin production caused by the decrease of activity and number of melanocyte and the accumulation of hydrogen peroxide (H2O2) in the hair follicle with increase of age. The purpose of this study was to investigate the effect the Black oryzasativa ethanolic extract (BLEE) on the melanin production. In this study BLEE at 8 μg/ml or more showed a significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and reduction power. BLEE at 16 μg/ml or more showed promoted tyrosinase activity and melanin production. In addition BLEE scavenged intracellular H2O2 in 2',7'-dichlorodihydrofluorescein (DCF) fluorescence assay in B16F1 cells. However, western blot analyses displayed that BLEE decreased the expression level of catalase, but no effect on the expression level of tyrosinase, tyrosinase associated protein-1 (TRP-1), tyrosinase associated protein-2 (TRP-2) and microphthalmia-associated transcription factor (MITF) transcription factor involved in melanogenesis. Thus, the promotive effect of BLEE on melanin production is attributed to the increase of tyrosinase activity and the reduction of intracellular H2O2 level. In conclusion, BLEE played a key role in in promoting melanin production, which suggests that the BLEE could be applied as a potential functional material in the development of hair care cosmetics related to the promotion of melanin production for the growth of black hair.
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KEYWORD
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Black oryzasativa, B16F1, catalase, melanin, tyrosinase
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